Download ScanFlex™ Instruction Manual - Flexcell International Corp.

™
SCANFLEX
AUTOMATED SCANNING DEVICE
INSTRUCTION MANUAL
SCANFLEX 3.1.4
REVISION3.2
Flexcell® International Corporation, 2730 Tucker Street, Suite 200 Burlington, NC 27215, USA Tel: 919‐732‐1591, Fax: 919‐732‐5196, www.flexcellint.com FLEXCELL®INTERNATIONALCORP
TableofContents
1.
Introduction.....................................................................................................................................................................2
1.1
UseofSCANFLEX™SoftwarewithTissueTrain®CulturePlates........................................................2
1.2
UseofSCANFLEX™SoftwareforOtherApplications...............................................................................2
2.
Components.....................................................................................................................................................................2
3.
InstallingtheScannerSoftware...............................................................................................................................3
4.
InstallingSCANFLEX™Software.................................................................................................................................4
5.
ActivationofSCANFLEX™Software..........................................................................................................................6
6.
ScannerSetup..................................................................................................................................................................6
7.
6.1
GeneralSettings....................................................................................................................................................6
6.2
ScanningTissueTrain®CulturePlates........................................................................................................7
SettingupSCANFLEX™Software................................................................................................................................9
7.1
OpeningSCANFLEX™Software.........................................................................................................................9
7.2
ConfiguringScanner............................................................................................................................................9
7.3
CreatingaUserLogin..........................................................................................................................................9
7.4
CreatingScanningRegimens.........................................................................................................................10
7.4.1
CreatingaNewRegimenforaNewUser........................................................................................10
7.4.2
CreatingaNewRegimenforanExistingUser..............................................................................12
7.4.3
UsinganExistingRegimen...................................................................................................................12
7.5
8.
ImageAcquisition...............................................................................................................................................13
Appendix..........................................................................................................................................................................17
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1. Introduction
1.1 UseofSCANFLEX™SoftwarewithTissueTrain®CulturePlates
SCANFLEX™softwareisdesignedtoautomaterepetitivescanningprocesses.Itwasinitiallydesigned
to scan 3D bioartificial tissue constructs fabricated using Flexcell’s Tissue Train® Culture System.
The SCANFLEX™ scanner and software enable the user to create scanning regimens so bioartificial
tissuesin6‐wellor24‐wellTissueTrain®cultureplatescanbescannedperiodicallytodetermine
the rate of matrix compaction by cells overtime. Once the scanning is completed, the series of
picturescanbeanalyzedforareameasurementusingFlexcell’sXYFLEX™software.TheSCANFLEX™
softwareallowstheusertocreatescanningregimensandsavethescannedimagestoapreferred
locationinadesktoporlaptopcomputer.
1.2 UseofSCANFLEX™SoftwareforOtherApplications
SCANFLEX™ software can also be used for any other application requiring periodic scanning. The
component to be scanned can be placed in a flat container or on the bed of the scanner. The
distancebetweenthescannerbedandtheitem(s)tobescannedshouldbenomorethan29mm.It
isnotrecommendedtokeepextremelyhotorcolditemsonthescannerbedasitcoulddamagethe
glass.
2. Components







SCANFLEX™softwareV3.1.4
EpsonPerfectionV300scanner
Framefor6‐wellcultureplates
Framefor24‐wellcultureplates
PlateCover
Usermanual
Desktopcomputerorlaptopcomputer(notprovided).SeeTable1formoredetails.
Table1.MinimumsystemrequirementsforSCANFLEX™andscanneroperation
Minimumsystemrequirements:USB2.0/1.1
CPU
RAM
MicrosoftWindowsXP,Vista,andWindows 7(32‐bit)
1GHz
1GB
MicrosoftWindowsXP,Vista,andWindows 7(64‐bit)
1 GHz
1GB
Minimumscreenresolution
1024×768pixels
HDD
95 MB
95 MB
Note:Theabovearetheminimumsystemspecificationsforthecomputertooperatethescanner.More
memorywillbeneededdependingonscannerfrequencyandimageresolution(seeTable3formore
informationonimagesizecorrespondingtoimageparametersettings).
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3. InstallingtheScannerSoftware
Thescannersoftwareisprovidedwiththescanner.Followtheinstructionsbelowtoinstallthe
scannersoftwareonthecomputer.
1) ItisrecommendedtosupplypowertothescannerandconnecttheUSBcablebetweenthe
computerandscannerbeforeinstallingthescannersoftware.
2) InserttheCDthatcamewiththescannerintotheCD‐ROMdriveofthecomputer.
3) Theinstallationwizardwillautomaticallyopen.Choosethedesirablelanguage.
4) Makesurethatantivirussoftwareisdisabled.ClickNEXTinthemessagescreen(Figure1).
5) Selectthescannermodel:EpsonPerfectionV300Photo(Figure2).
6) ClickINSTALLonthemoduleselectionscreen(Figure3).
7) OntheEpsonlicensescreenclickYEStoproceedwiththeinstallation(Figure4).
8) Thescannerinstallationwillbecompletedinafewminutes.
Figure1.Antivirussoftwarewarningduring
scannersoftwareinstallation
Figure2.Selectionofscannermodel
Figure4.Epsonlicenseagreement
Figure3.Selectionofmodules
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4. InstallingSCANFLEX™
Software
1) InserttheSCANFLEX™CDintotheCD‐
ROMdriveofthecomputer.
2) The SCANFLEX™ installation process
shouldstartautomatically.
3) Select the destination directory to
saveallprogramfiles(Figure5)and
clickNEXTtocontinue.
4) Click NEXT on the start installation
screen(Figure6).
5) Once installation is completed, click
on NEXT in the “installation
complete”screen(Figure7).
6) In the NI activation wizard screen,
select the first activation option
(Automatically activate through a
secure internet connection) and click
onNEXT(Figure8)
7) Enter the NI Vision Development
Module Run‐Time License number,
whichisincludedwiththeequipment
documentation, in the white box and
clickNEXT(Figure9).
8) Selectlanguageinthenextscreenand
clickOK.
9) TheEZTwainXsetupwizardwillstart
installing. On the EZTwainX setup
screen, click NEXT to proceed to
installation(Figure10).
10) On the EZTwainX license agreement
screen,select“Iaccepttheagreement”
andclickonNEXT(Figure11).
11) Select destination location for the
EZTwainX files to be saved
(Figure12).
12) Click on INSTALL on the next screen
tostartinstallation(Figure13).
13) Once installation is completed, click
onFINISH.
SCANFLEX™INSTRUCTIONMANUAL
Figure5.Selectingdestinationdirectory
Figure6.Installationstartscreen
Figure7.SCANFLEX™installationcompletescreen
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Figure8.Optionsforvisionmodulelicenseactivation
Figure10.EZTwainXsetupscreen
Figure9.Screenforenteringvisionmodulelicense
Figure11.EZTwainXlicenseagreement
Figure12.SelectinglocationtostoretheEZTwainX
files
Figure13.EZTwainXreadytoinstallscreen
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5. ActivationofSCANFLEX™Software
1) Click on the SCANFLEX™ icon on the desktop or go to program files and open SCANFLEX™
software.
2) When the SCANFLEX™ activation screen opens up, select either ACTIVATE SCANFLEX or
EVALUATESCANFLEX(Figure14).
3) IfACTIVATIONisselected,twousercodeswillbedisplayedonthenextscreen(Figure15).
Call (1‐800‐728‐3714 or 919‐732‐1591) or email (activation@flexcellint.com) Flexcell®
Internationalwiththeusercodestoobtainanactivationcode.
Note:DoNOTclosetheSCANFLEX™softwareuntiltheactivationcodeshavebeenreceivedfrom
Flexcell®International.Doingsowillgeneratenewusercodes,voidingtheprevioususercodes
andtheactivationcode.
4) EntertheactivationcodeinACTIVATIONCODE1boxandclickonCONTINUE.
5) If EVALUATE SCANFLEX is selected, you will have 30 days to use the SCANFLEX™ software
afterwhichitwillautomaticallydeactivate.
Figure15.Usercodeandactivationcodefor
SCANFLEX™activation
Figure14.OptionsforactivatingSCANFLEX™software
6. ScannerSetup
6.1 GeneralSettings
Plug in the scanner power cable to a 100‐240 VAC power outlet. Connect the USB cable that is
providedwithscannerbetweenthescannerandthecomputer(Figure16).Adedicatedcomputer
isrecommendedtousewiththeSCANFLEX™software.Thecomputershouldalsohavethefollowing
settingsturnedOFFforlongtermexperiments:
 Autoupdates
 Virusscanning
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 Sleepmode
 Automaticdefragmentation
ThescreensavershouldbeturnedONsuchthatascreenburn‐inorghostimagingwillnotoccurif
the scanner is used for especially long time periods. The maximum number of scans that can be
generated by the SCANFLEX™ software is only limited by the memory capacity of the computer to
storethescannedimages.Theprogramwillgiveanerrormessageandpausescanningifthereisno
longer space to store the scanned images. Always be certain that you have sufficient memory
capacity for all scanned images. The maximum number of images that can be stored for a given
amountofmemorycapacitycanbeincreasedbychoosingalowerimageresolution(seeTable3).
6.2 ScanningTissueTrain®CulturePlates
Twodifferentplateframes,oneframeforfour6‐wellplatesandoneframeforfour24‐wellplates,
are provided with the SCANFLEX™ software package (Figure 17). Place the corresponding plate
frame on the scanner making sure that it firmly fits within the scanner area. The frame has
markings on its side to identify the location of each culture plate (see Important: Note #1). The
culture plate location in the frame corresponds to the numbering of the plates and wells in the
XYFLEX™software.Iftheplatesareplacedaccordingtotheinstructions,thewellswillbenamedas
showninFigures18‐21intheXYFLEX™software.Seethe Appendixfor anillustrationtoidentify
the corresponding location of wells on the scanned image with reference to their physical
orientationonthescannerbed.
Power Adaptor
Computer USB cable
Scanner
Figure16.Scannerandcomputersetup. NOTE#1:THEPOSITIONOFTHE
PLATEFRAMETRIANGLESMUSTBE
POSITIONEDCLOSESTTOTHE
POWERBUTTONONTHESCANNER
FORTHEWELLLOCATIONSDEFINED
INFIGURES18THROUGH21TOBE
ACCURATE.
Figure17.ArrangementofFlexcell’sA)24‐wellandB)
6‐wellcultureplatesonthescannerbed.
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Figure19.Theorientationandnumberingof6‐well
Figure18.Theorientationandnumberingof24‐well
cultureplatesonthescannerwhentheframeprovided cultureplatesonthescannerwhentheframeprovided
isusedforplacingthecultureplatesonthescanner.
isusedforplacingthecultureplatesonthescanner.
Theblackareaistheframeusedtoholdtheplateson
Theblackareaistheframeusedtoholdtheplateson
thescannersurface.
thescannersurface.
Figure20.Theorientationandnumberingof24‐well
cultureplatewellsinthescannedpicture.Theblack
areaistheframeusedtoholdtheplatesonthe
scannersurface.Thisisthenumberingsystemwhichis
usedintheXYFLEX™softwareforidentificationofwells.
Figure21.Theorientationandnumberingof6‐well
cultureplatewellsinthescannedpicture.Theblack
areaistheframeusedtoholdtheplatesonthe
scannersurface.Thisisthenumberingsystemwhichis
usedintheXYFLEX™softwareforidentificationofwells.
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7. SettingupSCANFLEX™Software
7.1 OpeningSCANFLEX™Software
When the SCANFLEX™ software is completely installed, the
SCANFLEX™ icon (Figure 22) is displayed on the desktop. Click on
the icon to open the SCANFLEX™ software. The SCANFLEX™ start‐up
screen(Figure23)willopenup.
Figure22.SCANFLEX™icon
7.2 ConfiguringScanner
1) To configure the scanner, click on the
CONFIGURE SCANNER button in the
SCANFLEX™start‐upscreen(Figure23).
2) If the scanner is not already displayed
(Figure24),clickontheSELECTbuttonto
open‐uptheSelectSourcescreen.
3) If the scanner is properly installed, it
should be displayed in the Select Source
screen. Click on the correct scanner and
clickSELECT(Figure25).
4) If you do not see the scanner in the Select
Source screen, check the USB cable for
proper connection and power to the
scanner.Youmayalsohavetoreinstallthe
scannersoftware.
5) OntheSelectScannerscreen,clickSAVEto
savetheselectedscannerforscanning.
Figure23. SCANFLEX™start‐upscreen
Figure24. Scannerselectionscreen
7.3 CreatingaUserLogin
1) To access the SCANFLEX™ User Login
screen, click on START on the SCANFLEX™
Start‐upscreen(Figure23).
2) Click on CHOOSE USER to open‐up a drop
downmenu(Figure26).
3) If a new user has to be added, select ADD
USER from the drop down menu and type
the user’s name in the USER NAME box
(Figure27).ThenclickonCONTINUEtogo
tothesetupscreen.
SCANFLEX™INSTRUCTIONMANUAL
Figure25.Selectionofscannermodelscreen
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4) If the user’s name is already in
thelist,clickontheuser’sname
and then click CONTINUE. You
will be directed to the folder
which contains all previously
created configuration files
(regimens).
NOTE #2: Deleting a user from USER LIST If a user’s name needs to be deleted from the user list, click on the user name and then click DELETE. Click YES or NO on the popup warning message to select whether you want to delete all regimens that belong to this user. If you select NO, the user’s name will not be deleted from the list. If YES is selected, all regimens saved under that user will be deleted with the user name. Figure26.Choosingauser
Figure27.Addinganewuser
7.4 CreatingScanningRegimens
7.4.1 CreatingaNewRegimenforaNewUser
All new users will be directed to the SCANFLEX™ Setup screen. Follow the instructions below to
setupascanningregimen.
1) SetthescanningfrequencytimeunderEVERY,andselectunitsinTIMEBASE(Figure28,red
circle).Important:Themaximumscanningfrequencyisonescanperevery2minutes.The
minimumfrequencyisonescanperevery200hours.
2) SetSTOPTIMEbysettingdays,hours,minutesandseconds(Figure28,yellowrectangle).
3) ClickINSERTBELOWtoinsertfrequencydatatoStep#1inthetable.
4) Ifanotherstepneedstobesetup,entertherequiredparametersinEVERY,TIMEBASEandSTOP
TIMEandthenclickINSERTBELOW.TheStep#2willbedisplayedinthetable.RefertoNote#2
belowforexplanationonSTOPTIMEcalculation.
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5) Whenallstepshavebeenadded,
save regimen by clicking on
SAVE CONFIGURATION. A folder
withtheuser’snameas thetitle
willbeopened(Figure29).
6) Typethedesiredfilenameinthe
FILENAMEboxandclickonOK.
7) On the SCANFLEX™ Setup screen
(Figure 30), click on CONTINUE
to proceed to the image
acquisitionscreen(Figure31).
RefertoNote#4toaccessthe
locationwherethe
configurationfilesaresavedin
thecomputer.
NOTE #3: Calculating STOP TIME The Stop Time in the table displays the cumulative time. To calculate the time parameters that need to be entered under STOP TIME, add the absolute time for the next subsequent step to the time displayed under the current step. For example, assume that another step needs to be added in addition to the Step #1 shown in Figure 28 with a scanning frequency of every 2 minutes and a stop time of 30 minutes. Since Step #1 has 1 hr and 30 minutes, the stop time that needs to be entered for Step #2 is 2 hrs (1 hr 30min + 30 min). There will be 15 scans for Step #2. Figure28.Creatinganewregimen
Figure29. Savinganewregimen
NOTE #4: Access to configuration files The configuration file is saved in the “config” folder in the C drive. You may use the following address to locate the configuration files. To access, click START on your desktop and click on “My Computer”. In the top address bar type in the following address: C:\Documents and Settings\All Users\Application Data\Flexcell_Scanflex\config SCANFLEX™INSTRUCTIONMANUAL
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7.4.2 CreatingaNewRegimenforanExistingUser
Usethefollowinginstructionstocreateanewregimenforanexistinguser.
1) When the user name is selected
in the login screen (Figure 27),
the folder containing saved
regimen files (Configuration
files) will be opened as a popup
screen(Figure30).
2) ClickonCANCELtoexitfromthe
popupscreen.
3) Set the scanning frequency time
underEVERY,andselectunitsin
TIME BASE (Figure 28, red
circle).
4) Set STOP TIME by setting days,
hours, minutes and seconds
(Figure28,yellowrectangle).
Figure30. Openingexistingregimenfiles
5) Click INSERT BELOW to insert
frequencydatatoStep#1inthetable.
6) Ifanotherstepneedstobesetup,entertherequiredparametersinEVERY,TIMEBASEandSTOP
TIMEandthenclickINSERTBELOW.TheStep#2willbedisplayedinthetable.RefertoNote#3
aboveforexplanationonSTOPTIMEcalculation.
7) Whenallstepshavebeenadded,saveregimenbyclickingon SAVECONFIGURATION.Afolder
withuser’snameasthetitlewillbeopened(Figure31).
8) TypethedesiredfilenameintheFILENAMEboxandclickonOK.
9) OntheSCANFLEX™Setupscreen,clickonCONTINUEtoproceedtotheimageacquisitionscreen
(Figure31).
RefertoNote#4toaccessthelocationwheretheconfigurationfilesaresavedinthecomputer.
7.4.3 UsinganExistingRegimen
1) Foranexistinguser,whenthenameoftheuserisselectedfromtheUserlist(Figure27),the
foldercontainingallregimenfiles(Figure31)thatbelongtotheuserwillopenautomatically.
2) To load the desirable regimen file onto the SCANFLEX™ setup screen, click on the desired
RegimenFileandthenclickonOK.
3) Theselectedregimendatashouldthenbedisplayedintheparametertable.
4) Ifthedisplayeddataneedstobechangedormoredataneedstobeadded,usetheappropriate
buttonsdisplayedontheleftandbottomofthesetupscreen.Adescriptionofbuttonfunctions
isshowninTable2.
5) Ifanotherregimenfileneedstobeuploaded,clickontheLOADCONFIGURATIONbuttononthe
setupscreen.
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6) Inthepopupuserfolderscreen,selecttheappropriatefileandclickOKordoubleclickonthe
appropriatefile.
7) ClickonCONTINUEtoproceedtotheimageacquisitionscreen(Figure31).
RefertoNote#4toaccessthelocationwheretheconfigurationfilesaresavedinthecomputer.
Table2.FunctionsofthebuttonsinSCANFLEX™setupscreen
Button
Function
LoadConfiguration
Opensaregimen(configuration)filefromauserspecifiedlocation
SaveConfiguration
Savesaregimenfileintheuserconfigurationfolder
ClearTable
Clearsalldataintheregimentable
ModifyRow
Modifiescurrentdataintheregimentableaccordingtothe
frequencyandstoptimedatasetinthecurrentsetupscreen
InsertAbove
Insertsregimendataabovetheselectedrowinthetable
InsertBelow
Insertsregimendatabelowtheselectedrowinthetable
DeleteRow
Deletestheselectedrawinthetable
Continue
Continuestothenextscreenwhichistheimageacquisitionscreen
7.5 ImageAcquisition
The image acquisition screen
(Figure 31) enables the user to
set several parameters such as
image file type, quality, color
scheme, and resolution. It also
displays real time scanning
information such as the number
of scans completed, next scanning
time,andelapsedtime.Theimage
of the last scan is displayed on
the right side of the screen.
Follow the instructions shown
belowtoseteachparameter.
Figure31. Imageacquisitionscreen
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1) Basefilename
Enterafilenamethatwillbeusedasthebasefilenameforallyourstoredimages.Theimages
willbenamedusingthistextasthebasewithatimestamp.Forexample,ifthebasefilenameis
Test, all images will be saved in the format “Test‐0000hr00min” sequentially according to the
scannedtime.
NOTE:Anewimagefolderneedstobeopenedifthesamebasefilenameneedstobeused
forimagesinanewscanningregimen.Changethebasefilenameifthenewimagesneedto
be stored in the same folder. Otherwise, the images will not be stored, and the SCANFLEX™
softwarewilldisplayanerrormessage.
2) Imagefolder
Click on the image folder icon (Figure 31, red circle) to specify a location for the scanned
imagestobestored.Thefilepathoftheselectedlocationwillbedisplayedintheimagefolder
box.
3) Filetype
ClickontheFILETYPEboxtoselecteitherBMPorJPGforthefiletypeofthescannedimage.
4) Quality
Thequalityoftheimagecanbevariedfrom0to100inincrementsof5.Thehigherthequality
of the image, the larger the file size will be. The relationship between the quality setting and
imagefilesizeisshowninTable3.Usetheup/downbuttonstosetthequalityoftheimage.
Table3.Relationshipbetweenimagefilesizeandimageparametersetting.
Imagefiletype
BMP
Resolutionsetting(dpi)
600
JPG
600
BMP
300
JPG
300
BMP
150
JPG
150
Qualitysetting(%)
N/A
100
75
50
25
N/A
100
75
50
25
N/A
100
75
50
25
Imagesize(MB)
102
19.2
2.96
1.96
1.29
25.6
5.53
0.93
0.62
0.39
6.4
1.44
0.28
0.19
0.13
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5) Colorscheme
Thecolorschemeoftheimagescanbesettooneofthefollowing:
 1bitB&W
 8bitGrayscale
 24bitcolor
Clickontheboxandchoosethedesiredcolorsetting.
6) Resolution
Theresolutionofthescannedimagecanbesettooneofthefollowing:
 150dpi
 300dpi
 600dpi
Thesizeoftheimagefileandthescantimewilldependontheselectedresolution(seeTables3
and4).Clickontheboxtochoosethedesiredimageresolution.
7) Frequency
The scanning frequency set in the SCANFLEX™ setup screen will be displayed in the frequency
display.
8) Scanscomplete
Thisboxwilldisplaythetotalnumberofscansandthenumberofscanscompletedatagiven
time.
9) Nextscan
This box will display the time left for the next scan. This time is based on the scanning
frequencyvaluesetinthesetupscreen.Itwillcountdownthetimeforthenextscanoncethe
STARTbuttonisactivated.NOTE:Thetimerwillalsocountthetimespentforscanning.Table4
illustratesthescantimefordifferentimageresolutionsettings.
Table4.Scantimefordifferentimageresolutions
Imageresolution(dpi)
150
300
600
Scantime(sec)
27
33
57
10) Elapsedtime
Theboxwilldisplaytheamountoftimepassed,countingupfromthefirstscan.Itwillalsotake
into account the time spent on scanning the image. If multiple steps are setup in the regimen
table,theelapsedtimewilldisplaythetotaltimepassedoncetheSTARTbuttonisactivated.
11) Onceallscannerparametersarecorrectlyset,clickonPREVIEWtogenerateapreviewscanof
theitems.Makesuretheitemstobescannedareinthecorrectorientationonthescannerbed,
andthescannerlidisplacedontheitemspriortogeneratingapreview.
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12) Reviewthepreviewimage.Iftheimageiscorrect,clickonSTARTtobeginthescanningprocess.
Thescannerwillstartgeneratingimagesaccordingtothesetregimen.
13) Ifapreviewisnotnecessary,clickSTARTtobeginthescanningprocess
14) If any changes need to be done to the scanner parameters once the scanning process has
started,clickonSTOPandthenmakenecessarychangestotheparameters.Repeatsteps11‐13
torestartscanningprocess.
15) If any changes need to be done to the scanning regimen, click on STOP and then SETUP to go
back to the SCANFLEX™ setup screen. Repeat procedures shown under Section 7.4.1, 7.4.2, or
7.4.3 as necessary to setup a scan regimen. Recheck the scanner parameters on the image
acquisitionscreenpriortostartingscanning.
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8. Appendix
A B
Figure32.PhysicalorientationofthewellsinFlexcell’s24‐wellplatesonthescannerbed(A)andtheir
correspondinglocationsinthescannedimage(B),
A B
Figure33.PhysicalorientationofthewellsinFlexcell's6‐wellplatesonthescannerbed(A)andtheir
correspondinglocationsinthescannedimage(B),
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