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TITLE:
OPTRONICS User Manual
P. Grosbol , ESO - Garehing
June 16 , 1981
PURPOSE: User guide for the O~ICS rneasuring machine
C. Euler , Roorn no, 222
ID::
User
TYPE:
AtJrHOR:
REV:
CHAP.I'ER 3
OPERATION OF OPI'RONICS
3.1
INI'RODUC!'ION
Preparing the microdensitometer for operation involves rnany
steps which can be performed in rnany different ways. It is
suggested, however, to follow the steps as outlined in this
section.
Only operations of the microdensitometer is described
in this manual while instructions for operation and running the
scan control programs are found in the Compute Prograrn section pf
the manual.
Note that all functions of the OPrroNICS machine are
controled by the satellite computer. Therefore, it is essential
that the satellite control prograrn is loaded and running.
If
same functions are not working properly try to run the prograrn
'READY' and reload the satellite computer.
3.2
1.
2.
3.
4.
S.
3.3
1.
2.
STARTUP PROCEDURE
Check that the power is on to the system rack and the
microdensitome The X/y coordinate display on the rack should
be lit.
Check that the microdensitorneter Larep is lit.
If not call
for help!
Run the control prograrn 'OPrro' or the user application
program on the terminal.
Switch the ON/STANDBY switch to ON by pressing the ON button
on the main control panel.
Check that the stage can rnove in both X and Y direction by
pressing the X/Y positon buttons on the main control panel.
If it cannot be moved call for help!
CIDSElXMN PROCEDURE
Terminate the control program.
Switch the lamp switch on the system rack to NORMAL position.
OPERATION OF OPl'RONICS
Page 3-2
3.4 OPrICAL CALIBRATION
The optical calibration and adjustment consist of rnounting
p1ate, selecting slit and aligning optics. The two 1atter items
are not needed when only manual measurements are made.
3.4.1 Plate Mounting
1.
2.
3.
4.
5.
For the most corranonly used plate sizes special adapters are
available ( see Table 2.5 ). If other p1ate sizes are used a
glass platen should be placed in the plate adapter.
Move the stage in the Y-axis towards the main control panel
stopping it approxirnately 10 cm before the end.
Attach the plate adapter to the stage and fasten it using the
clip and thumbscrew in the corner ,
Each adapter has three reference dowel pins and a clip in one
corner , Place the p1ate in its adapter and align against the
reference dowe1s. Use the clip to push the plate against the
dowles.
Tighten the thumbscrew on the clip to secure the plate in its
adapter.
3.4.2 S1it Selection
1.
2.
3.
4.
5.
Find
the
best eyepiece / objective / slit combination
yielding the scanning slit desired ( see Table 3.1 ). Note
that norrnally the 5x objectives will be mounted, and that
objectives only will be changed in special cases ( contact
staff member in charge ).
Se1ect the lower eyepiece on the lower control panel by
turning the MAGNIFICATION knob.
Install the upper eyepiece in the upper microscope.
Its
magnification must be identical, to that chosen for the lower
eyepiece. A change is done by bringing the microscope to
full down position, unscrewing the top part, taking it out,
replacing the eyepiece and remounting the top part.
Select the pres1it on the lower control panel using the FIXED
APERrURE knob,
Match the corresponding slit on the slit-wheel (i.e.
the
leftmost wheel) inside access door on the rigth side of the
brigde. The slit number pointing upwards on the slit-wheel
should be identical to the preslit number selected on the
lower control panel.
3.4.3 Optical Alignment
1.
2.
3.
Engage field illuminator by turning ILLUMINATION knob on the
lower contro1 panel to IN position.
Focus the plate on the viewing screen by using the focus knob
on the upper microscope.
Check that a good focus is obtained over the entire area
OPERATION OF OPl'RONICS
Page 3-3
which has to be scanned by moving the stage rnanually to
different positions on the plate.
If this not the case
adjust the -horizontal level of the stage using the two
micrometric screews below the stage ( one on each side ).
4. Remove the cape from the telescope to the left of the viewing
screen.
Focus the telescope by pulling a little out. The
slit aperture should now be sharp.
5. Switch the ILLUMINATION knob on the Iower control panel to
OUT position.
6. Focus the preslit on the viewing screen using the u:MER FOCUS
buttons on the main control Panel.
Since the focus is
adjusted in very small steps it may take some time before a
goOO focus is reached. It is easier to determine the best
focus when the plate is positioned on an area with low
density.
.
7. Check that the preslit is positioned close to center of the
viewing screen crosshair.
If this is not the case ask the
staff member in charge to adjustithe upper microscope body.
8. Use the viewing telescope to the left of the screen to center
the preslit and the main slit. Alignment is accomplished by
turning the knobs inside access door on the right side of the
bridge.
The first knob adjust the X-axis, the next the
Y-axis, and the third the rotation.
9. When not in use place the telescope cap on the telescope to
avoid that roan-light can reach the PMT.
3.5 ANALOO SYSTm CALIBRATION
The calibration of the analog system must be done with the
ILLUMINATION knob on the lower control Panel in OUT position.
The microdensitometer spot should be positioned on the area of
the plate which should be defined as density 0.0 or transmission
1.0 (e.g. clear glass plate or plate fog ). It must be noted
that
the OPTRONICS does not measure absolute density or
transmission values. Since the ca1ibration is sensitive to any
in
the optica1 system (e.g.
refocusing) a new
change
ca1ibration have to be performed when ever such changes are made.
3.5.1 Transmission Mode
1.
2.
3.
4.
5.
6.
Select transmission mode on the main control panel by
pressing the DENS button until the TRANS control lamp is lit.
Check that the ILLUMINATION knob on the lower control panel
is in OUT position.
Place a dark piece of paper on the plate so that it obscures
the light beam. Close the viewing screen and set the cap on
the telescope.
Adjust the ZERO LEVEL potentiometer on the main control Panel
for a DIGITAL METER reading of 5 units.
Remove the paper from the plate.
Position the plate on the area of the plate which should be
defined as transmission 1.00.
The viewing screen may be
opened.
OPERATION OF OPTroNICS
7.
Page 3-4
Select a neutral filter combination to obtain the lagest
Mm'ER reading less or equal to 4050 units ( the I F I command
in the control program may be used to get the best setting ).
The
neutral filters are ·selected by rotating the two
rightmost wheels inside the access door at the right side of
the bridge.
If a sufficiently high METER reading cannot be
,reached the larnp switch on the rack may be switched to MAX
position.
3.5.2 Density Mode
1.
2.
3.
4.
5.
6.
7.
3.6
Select density mode by pressing the DENS button until it is
lit.
Check that the ILLUMINATION knob on the lower control panel
is in OUT position.
Place a dark piece of paper on the plate so that it obscures
the light beam. Close the viewing screen and set the cap on
the telescope.
Adjust the ZERO LEVEL potentiometer on the main control panel
for a DIGITAL METER reading of 5 units.
Remove the paper. from the plate.
Position the plate on the area of the plate which should be
defined as density 0.0. The viewing screen may be opened.
Select a neutral filter combination to obtain the largest
METER reading less or equal to 4050 units ( the 'F ' cornmand
in the control program may be used to get the best setting ).
The neutral filters are selected by rotation of the two
rightmost wheels inside the access door at the right side of
the bridge.
If a sufficiently high METER reading cannot be
reached the larnp switch on the rack may be switched to MAX
position.
SCANNING
After the optical and analog systems
are
calibrated
measurements can be made.
The telescope and viewing.screen
should be covered when the machine is measur ing •
Scans can be
performed· by running the proram I OPTRQ I (either online or
offline) or a user application program on the central computer.
These programs are described in the Computer Program section of
the manual.
OPERATION OF OPI'RONICS
Page 3-5
Table 3.1
Scanning
aper~ure f~"micron
5x objective
lex objective
--Aperture
Eyepiece
15x
2ex
'5
" 3.3 x 3.3
2.5 x 2.5
6.7 x 6.7
lex
5x
1
Hl x
leJ
5
2
213 x 20
10
~x
10
3
30 x 30
15
x
15
. .'."
Xt
4
513 x 50 "" 25
x 25
5
15 x 140
7~5
x 713'
6
10 x 140
5
x
7
15 x 280
8
10 x 280,
5
x 5
x 10
7.5 x 7.5
17' ' x 17
12.5 x 12.5
lß
5
x 42
3.8 x 35
3~3
x 42
2.5 x 35
"7 ~5x 1213
5' x 813
3.8 x 60
5 ' x 120
3.3 x 80
2.5 x 80
713