Download GeneQuant User Manual

CONTENTS
Page
Section
2
INTRODUCTION
3
SYMBOLS
3
REAR PANEL
4
BEFORE INSTALLATION
4
INSTALLATION
5
6
CALCULATION KEYS
Other Keys
7
8
9
10
11
OPERATION
Instrument Set Up
Reference Measurement
Sample Measurement
Printer Output Version
15
15
15
16
16
ACCESSORIES AND CONSUMABLES
Selecting the Appropriate Cell
UV/Visible Cell Order Information
Using Cells
Other
17
FACTORS AND FORMULAE
18
18
18
19
MAINTENANCE
Cleaning and General Care
Fuse Replacement
Deuterium Lamp Replacement
20
SPECIFICATIONS
Issue 03 - 01/2000
English
1
INTRODUCTION
GeneQuant,theRNA/DNAcalculator,isaspectrophotometer designed
specificallyformolecularbiologists.
The instrument measures RNA and DNA samples in UV cells at 230 nm, 260 nm,
280 nm and 320 nm simultaneously. The wavelengths 260 nm and 280 nm are
usedforquantificationandpuritycheckingcalculations,whereas320nmcanbe
used for background compensation. 230 nm can be used as a guide for protein
determinationusingthepeptidebondabsorbance. Aftereachsamplereadingthe
informationisstoredandusedincalculationsuntilthenextreadingistaken.
GeneQuantcanbeleftoncontinuouslywithoutaffectingthelifetimeofthelight
sourceandthisisrecommendedfortheconvenienceoftheusertoobtainrapid
measurements. Thenormalstatusofthelampisstandby modeanditisonly
powered up during actual reference and sample measurement using a unique
demandswitchingtechnique. (Patentappliedfor).
YoucanuseGeneQuanttocalculate:
o
RNA, ssDNA and dsDNA concentrations in units of weight, molar fraction,
molesofphosphateandtotalmolecules
o
A260/A280 ratio
o
Totalproteinconcentration
o
Recoveryofoligonucleotides
o
Purityofnucleicacids
o
Meltingtemperature
o
Molecularweightofoligonucleotides
2
English
Issue 03 - 01/2000
SYMBOLS
Symbols found in the manual:
Instrument
Ready
PleaseNote
sample
Warning
DisplayPanel
Key Press
Symbolsfoundontheinstrument:
!
Backgroundyellow,symbolblack.
Caution-refertoaccompanyingdocuments.
REAR PANEL
Mains Power
Switch
"I" = ON
"O" = OFF
Voltage
Selector
Cooling Fan
Outlet
PRINTER
LO 100 - 120V
HI 200 - 240V
Fuse
Holder
1.25A T LO
630mA T HI
Power
Input
Socket
Issue 03 - 01/2000
Printer Socket
(Printer Version Only)
English
3
BEFORE INSTALLATION
Inspecttheinstrumentforanysignsofdamagecausedintransit. Ifanydamageis
apparenttheninformyoursupplierimmediatelyanddonotproceedwiththe
installation.
Checkthat:
o
o
theinstallationsiteconformstotheenvironmentalconditionsforsafe
operation(seeSpecifications).
thecoolingfanoutletisnotobstructed.
INSTALLATION
This equipment must be connected to the power supply with the power
supply cord provided and MUST BE EARTHED.
Ifthisequipmentisusedinamannernotspecifiedorinenvironmental
conditionsnotappropriateforsafeoperation,theprotectionprovidedby
the equipment may be impaired and instrument warranty withdrawn.
o
o
o
o
o
o
Selectthecorrectvoltageforyourlocalsupply,usingthevoltage
selectorontherearpanel.
Selecttheappropriatefusesforyourlocalsupply. Twoidentical
fuses need to be loaded. For LO 100-120V operation use
2 x 1.25A T fuses and for HI 200-240V operation use 2 x 630mA T
fuses. (SeeMaintenanceforfittingfuses).
Connectthepowersupplycordtotheinputsocketontherearpanel
andtothepowersupply.
Switchontheinstrument.
Foroptimumlocationofthecell,repositionthespringcliplocated
in the sample compartmentby pulling firmlyupwardsand
relocatinginappropriatecentralslotsandifyouarenotusing
10mmcellsresetthepathlengthin'setup'.
^Ifyouareusingaprinter,checkthatitisaparallelversionandis
switched'on-line'ifnecessary. Ensurethattheprinteroptionsare
selectedin'set-up'.
^appliestoprinteroutputversiononly
4
English
Issue 03 - 01/2000
CALCULATION KEYS
Ensureallparametersin'setup'areappropriateforyoursample.
abs
ratio
RNA
DNA
todisplayabsorbancereading.
Press'select'tocyclethroughthe4wavelengthvalues
230nm, 260nm, 280nm and 320nm.
**indicatesthatacellpathlengthof10mmhasnotbeenselected.
To convert to corresponding OD values for a 10mm cell, multiply by
2,10or20for5,1and0.5mmpathlengthcells,respectively.
todisplayA260/A280 absorbanceratiowithorwithoutbackground
correctionat320nm. See'setup'.
to display concentrations of RNA, dsDNA or ssDNA.
Press'select'tocyclethroughchoiceofunits:
Conc 1: µg/ml - range 1 - 4000µg/ml
Conc 2: µg/µl-range0.001-0.2µg/µl
Conc 3: pmol/µl-range0.001-200pmol/µl
(Note: ensurethe+ oligonucleotidelengthandfactor
valuearecorrect. See'setup')
Conc 4: Phosphateconcentrationpmol-range0.001-0.200pmol
protein
todisplayproteinconcentrationinmg/ml-range0-600mg/ml.
(Note: ensurethecoefficientsarecorrect. See'setup').
[x]
todisplaymolecules/ml.
(Note: ensurethecorrectmolecularweightisentered. See'setup').
purity
todisplaypercentagepuritybycomparingactualratiotoexpected
ratio. (Note: ensuretheexpectedratioisentered. See'setup').
recovery
tocalculatepercentagerecoverybycomparingactualtoexpected
concentration(pmol/µl).
(Note: ensuretheexpectedconcentrationandtheoligonucleotide
lengthareentered. See'setup').
+
doesnotapplytoprinteroutputversion
Issue 03 - 01/2000
English
5
Tm
tocalculatemeltingtemperature. ^Press'Select'tocyclebetween
shortoligonucleotideandprimercalculation.
(Note: ensurethatthenumberofbases and^molarityarecorrect.
See'setup').
'!!'indicatesthattheequationisnotstrictlyapplicableforthebase
numbersused. (SeeFactorsandFormulae).
Other Keys
factor
enter
select
set
ref
sample
f1
f2
to change factor values for RNA, ssDNA and dsDNA and select
thecurrentmodeforcalculations.
toenteravalueoroption.
tochooseavalueoroptionwithinafunction.
tomeasurereference.
tomeasuresample.
^toprintoutthelistof'set-up'options.
undefined-availableforfuturedevelopment.
^appliestoprinteroutputversiononly
6
English
Issue 03 - 01/2000
OPERATION
Togetstarted:
o
switchontheinstrumentattherearpanel
Instrument
Initialising
Note: Instrumentinitialisesforafewseconds.
Instrument
Ready
o
o
o
o
select'setup'values. Thisstepcanbeomittedforquickabsorbancereadings
setreference
insertthecellsothatthelightpathdirectionisinthefront-to-rearaxisofthe
instrumentasindicatedbythearrow
measure your sample
TheinstrumentcanbeuseddirectlyasacalculatorforTmand
molecularweightswithoutmeasurementofasample.
Asecurityfacilitywhichlockstheinstrumentkeypadisavailable.
When'InstrumentReady'oracalculationresultisdisplayed,keyin
4080toswitchfacilityon. Theinstrumentwillthendisplay'Instrument
Locked';keyin4080againtounlock. Theinstrumentremainslocked
throughpoweroff/on.
Calculationkeys,exceptTm,willonlyfunctionafterasample
measurementhasbeentaken.
Issue 03 - 01/2000
English
7
Instrument Set Up
Ifyoukeyinanumberincorrectly,press'C'andstartagain.
set-up
and
enter
tostepthrough'setup'optionsandentertheparameters
whichrelatetoyoursample. Toexit'setup'pressany
calculationkey. Toreturntothebeginning,press'setup'.
Thedefaultparametersusedintheinstrumentmaybealteredasfollows:
o
o
o
o
o
o
o
o
o
o
o
o
o
o
o
o
Cell pathlength (mm). Press 'select' to choose from: 0.5 1 5 10.
^Printer. Press'select'tochoosefromON/OFF. Press'enter'.
^Samplenumber. Keyintherequired number. Press 'enter'.
Thesamplenumberincrementsautomaticallyeachtimethesampleis
measured.
^Date. Keyinthedate. Press'enter'. (Adjustthisdaily).
^Month. Selectappropriatemonth. Press'enter'.
^Year. Keyintheyear. Press'enter'.
Backgroundcompensation320nm. Press'select'tochoosefrom: YES/NO.
^Dilute? Keyinthedilutionfactorforconcentrationcalculation.
Range1.00-99999.9.
Factor. Press 'select' to choose from: RNA dsDNA ssDNA.
ForsyntheticoligonucleotidesusessDNAandkeyinnewfactorsifdefaults
arenotsuitable.Press'enter'.
Bases. Key in the number of A C G T U to calculate molecular weight
frombasecompositionofnucleicacidsandtoshowresultsasmolecules/ml.
Press'enter'tocyclethroughthebases. Range0-1000.
Oligolength. + Keyintheoligonucleotidelengthofthesampleinbaseunits
toshowresultsaspmol/µl. Press'enter'. Range1-9999.
Molecularweight(MW). Press'select'tochoosebetweenthecalculated
value(fromA,C,G,T,Unumbers)oruser-enteredvalueformolecular
weight(ifknown). Press'enter'.
Ratio. KeyintheA260/A280 absorbanceratioexpectedforyoursample(if
known). Press'enter'.
Concentration. Keyintheconcentrationexpected(inpmoles/µl)foryour
sample(ifknown). Press'enter'.
Protein. Keyincoefficientsforequationiftheyaredifferenttodefaults.
(SeeFactorsandFormulae).
^Molarity. Keyinmolarityofsaltsinhybridisationsolution.
^appliestoprinteroutputversiononly
+
doesnotapplytoprinteroutputversion
8
English
Issue 03 - 01/2000
Reference Measurement
Takeareferencereading. Thisreadingisstoredandusedasthebasereadingfor
allsamplemeasurementsuntilanewreferencereadingistaken.
Ifyoudonotinsertand
removethesamplecell
intime,thedisplaywill
show:
1.
Remove Cell
Press Key Again
set
ref
Please
Wait
2.
Waitforthetoneandinsert
thereferencecellintothe
samplecompartment:
Insert
Reference
3.
Wait for the tone and remove
thereference:
Remove
Reference
4.
After you have removed the
referencethedisplaywillshow:
ABSORBANCE
260 nm
0.000 AU
Whenusingthecapillarycellandholder,placetheholderinthesample
compartmentbeforetakingmeasurements. Then insertand removethe
capillaryasabove.
Issue 03 - 01/2000
English
9
Sample Measurement
You can now measure a sample. A similar procedure is followed to the one used
formeasuringareference. Usethe'sample'keyinsteadof'setref'.
Ensurecorrectcellpathlengthandsampletypehavebeenselectedin'setup'.
1.
sample
Please
Wait
or
^
Date 23 Aug 1993
Sample No. 9164
2.
Waitforthetoneand
insertthesamplecellinto
thesamplecompartment:
Insert
Sample
3.
Waitforthetoneand
removethesamplecell:
Remove
Sample
4.
Afterthefirstsample,
thedisplaywillshow
absorbanceat260nm:
ABSORBANCE
260 nm
1.000 AU
'Absorbance**'indicatesthata10mm
pathlengthcellhasnotbeenselected.
(See'setup').
Storedreadingscannowbemanipulatedusingthecalculationkeys.
Forsubsequentsamplesthedisplaydefaultstothepreviousfunction
used.
Thisfacilityprovidesusefulsinglekeyoperationifyourequirethesame
typeofmeasurementonsuccessivesamples. Operationofthe'sample'
keyautomaticallydisplaystheresultaftersamplemeasurement.
^displayedonprinteroutputversiononly
10
English
Issue 03 - 01/2000
Printer Output Version
Thissectionshouldbereadinadditiontothedetailsgivenintheprevioussections.
Installation
o
o
Connecttheprinterviaitsinterfacecabletothesocketontherearpanelofthe
instrument.
Switchontheprinterandensureitison-line.
Operation
Thesamplenumberanddatearestoredpermanentlyuntilaltered.
Samplenumberisincrementedautomaticallyeachtimeasampleis
measured. Thedatedoesnotincrementautomatically.
Ifyoudonotwishtoalteranyfurtherparametersin‘setup’,takereference
readingsandsamplemeasurementsasdescribedpreviously. Thedisplaysand
printoutsappearasshownbelow(whentheprinteroptionin'setup'issetto'on',
printoutsofthedisplayoccurautomatically).
Afteryouhaveremovedthereference:
ABSORBANCE
260 nm
0.000 AU
Absorbance
260 nm 0.000 AU
select
ABSORBANCE
280 nm
0.000 AU
Absorbance
280 nm 0.000 AU
Theremainingabsorbancevaluescanbeprintedoutinthesameway.
Issue 03 - 01/2000
English
11
When a sample is measured:
sample
Date 23 Aug 1993
Sample No. 9164
Operator _______
Date 23 Aug 1993
Sample No. 9164
Insert
Sample
Remove
Sample
ABSORBANCE
260 nm
0.957 AU
Absorbance
260 nm 0.957 AU
RNA
DNA
dsDNA CONC 1
47.9 µg/ml
dsDNA CONC 1
47.9 ug/ml
Ifrequiredpress‘select’tocyclethroughandprintouttheotherchoicesofunits.
Othercalculationsareprintedoutautomaticallywhentheappropriatekeyis
pressed.
Theinstrumentfacilitatesroutineanalysisasthedisplayandprintout
alwaysshowtheselectedparametersautomaticallyaftera
measurement,as shownin theexampleson the followingpage.
Iftheprinterisswitchedofforoff-lineduringusewhileselected‘on’in‘setup’
andakeyispressed,thedisplayshows:
Printer off-line
Press any key
Normalfunctioningresumesafteranykeyispressed. Iftheprinterisswitchedon
again,normalprintingresumes. Iftheprinterremainsoff,theprinteroptionin'set
up'isautomaticallysettooff.
12
English
Issue 03 - 01/2000
Examples:
'setup'options
GeneQuant
Path Length
Printer
Sample No.
Date
Month
Year
Use 320 nm
Dilute?
FACTOR
ssDNA?
BASES
Number
Number
Number
Number
Number
SETUP
10
ON
507
18
Jan
1993
NO
1.00
37.0
A?
C?
G?
T?
U?
OLIGO
Length
5
5
2
1
1
CONCENTRATION
Expected?
Routineanalysis
ofproteins
Operator________
Date 18 Jan 1993
Sample No. 0505
Operator________
Date 23 Feb 1993
Sample No. 8273
ssDNA CONC 1
29.3 ug/ml
PROTEIN CONC
0.7 mg/ml
ssDNA CONC 2
0.029 ug/ul
Operator________
Date 23 Feb 1993
Sample No. 8274
ssDNA CONC 3
0.197 pmol/ul
Phosphate CONC
0.093 pmol
RATIO
1.997
PURITY
99%
13
MOLECULAR WEIGHT
CALC
3961.6
RATIO
Expected?
Completelistof
calculationsfora
nucleotide
PROTEIN CONC
0.4 mg/ml
Operator________
Date 23 Feb 1993
Sample No. 8275
PROTEIN CONC
1.5 mg/ml
RECOVERY
98%
Operator________
Date 23 Feb 1993
Sample No. 8276
MOLECULES/ML
4.459395 Exp 15
PROTEIN CONC
3.8 mg/ml
MELTING TEMP
40 C
Operator________
Date 23 Feb 1993
Sample No. 8277
1.800
2.000
MELTING TEMP PRI
48.5 C
PROTEIN
Coeff 1?
Coeff 2?
1.550
0.760
Molarity
0.100
PROTEIN CONC
32.2 mg/ml
Operator________
Date 23 Feb 1993
Sample No. 8278
PROTEIN CONC
0.2 mg/ml
Issue 03 - 01/2000
English
13
Parallel Printer Interface
Specifications:
Datatransmission:
Synchronisation:
8bitparallel
Timingforattachedprinterisprovidedbyexternalstrobe
signals.
Handshakeprotocol: By BUSY and ~STROBE signals.
Signallevels:
Thelevelsofoutputdataandinterfacecontrolsignalsareall
TTLcompatible.
Interface Connector
Theprinterinterfaceisastandard25-pinD-shellfemaleconnector. Thedatalines
(D0-D7) on the connector are driven by drivers capable of sourcing 15mA and
sinking 24mA.
PinNo.
1
2
3
4
5
6
7
8
9
10
11
12-17
18-25
14
I/O
O
O
O
O
O
O
O
O
O
I
I
N/A
N/A
Signal Name
~STROBE
DataBit0
DataBit1
DataBit2
DataBit3
DataBit4
DataBit5
DataBit6
DataBit7
~ACK
BUSY
Unconnected
Ground
English
Issue 03 - 01/2000
ACCESSORIES AND CONSUMABLES
Selecting the Appropriate Cell
GeneQuant has a suitable measuring range between 0.1 to 2.5 OD for a 10mm
pathlengthcell. Chooseasuitablecelldependingonsampleconcentrationrange,
dilutionfactorandavailablesamplevolume.
Pathlength,mm
Suitable Sample
OD260 range
µg/ml) given that
Concentration range (µ
µg/ml for dsDNA
1.0 OD260 = 50µ
10
5
1
0.5
2.5-0.1
5-0.2
25-1.0
50-2.0
125 - 5
250 - 10
1,250-50
2,500-100
UV/Visible Cell Order Information
Pathlength and Description
Minimum Sample Volume
Part Number
10mm,standardcellwithlid
10mm,semimicro cellwithlid
10mm,microcellwithlid
10mm,ultramicrovolumecell
2,000µl
500µl
250µl
70µl
80-2002-58
80-2002-77
80-2002-95
80-2103-69
5mm,standardcellwithlid
5mm,ultramicrovolumecell
1,000µl
5µl
80-2002-57
80-2103-68
1mm,standardcellwithlid
200µl
80-2002-54
0.5mm,quartzcapillarycell
(includes100capillaries)
Sparequartzcapillaries(100)
3µl
80-2104-66
3µl
80-2104-67
Issue 03 - 01/2000
English
15
Using Cells
Ensurethatthecellfacesarecleanbeforemeasurement. Afterusecellsshouldbe
cleanedwithadilutealkali(e.g.0.1MNaOH)andadiluteacid(e.g.0.1MHCl)
wash,followedbyrinsingseveraltimeswithdistilledwater. Morerigorous
cleaningafterdifficultsamplesshouldbeperformedwithasuitableliquid
detergent,followingthemanufacturer'sinstruction.
The0.5mmquartzcapillaryisfilledbydippingintothesample. Afteruse,itcan
beemptiedusingaPasteurpipettebulbattachedtonarrowboresiliconetubing.
Thequartzcapillarycellcanbedismantledforcleaningandremovingabroken
capillarybyunscrewingthetwoscrewsoneachsideusingthetoolprovided.
Instructionsareprovidedwiththe0.5mmquartzcapillaryand5mmultra
microvolumecells.
Other
GeneQuant
GeneQuant(printerversion)
GeneQuant II
Deuterium lamp
GeneQuant User Manual
GeneQuant II User Manual
Dust Cover
ParallelInterfaceCable(Centronics)
IntroductiontoBasicUV/VisibleSpectrophotometry
16
English
80-2103-98
80-2104-98
80-2105-98
80-2104-56
80-2105-20
80-2105-58
80-2105-18
80-2071-87
80-2005-60
Issue 03 - 01/2000
FACTORS AND FORMULAE
o
Grams(g)areconvertedtomolesusing309(theaveragemolecularweightof
the ATCG bases)
o
Factorsformolecularweights(MW)ofbases:A=312.2
C=288.2
G=328.2
T=303.2
U=289.2
o
Fordephosphorylatedoligonucleotidessubtract61fromthecalculatedMW
o
Forphosphorylatedoligonucleotidesadd17tothecalculatedMW
o
A260 xfactor=µg/mlconcentration
Default factors are: 40 for RNA, 37 for ssDNA, 50 for dsDNA
ForsyntheticoligonucleotidesusessDNAmodeandchangefactor
o
A260/A280 ratios are 1.8 and 2.0 for pure DNA and RNA preparations,
respectively.
o
pmol/µl=
o
pmolphosphate=Nucleotideconcentration,µg/ml
315
o
Molecules/ml= concentration,µg/mlx6.023x1023
MW x 106
o
Protein (mg/ml) = 1.55 x (A280 - A320) - 0.76 x (A260 - A320)
µg/ml x 1000
309xoligolength
^ pmol/µl = µg/ml x 1000
M W
Coefficients1and2(defaults1.55and0.76,respectively)canbechangedfor
differentproteins
o
Tm 1 (for short oligonucleotides) = 2 (nA + nT) + 4 (nG + nC), n refers to
numberofindividualbaseunits-equationisvalidlinearlyfrom10to18mer,
but may be used as a guideline for values above 18 mer.
o
^ Tm primers = 81.5 + 16.6 (log molarity) +
0.41(%guanosine+cytosine)-500/primerlength
Equation is valid from 14 to 60 mer
^appliestoprinteroutputversiononly
Issue 03 - 01/2000
English
17
MAINTENANCE
Observeallnecessaryprecautionsifdealingwithhazardoussamplesor
solvents.
Usermaintenanceisrestrictedtochangingtheinstrumentlamp,changingexternal
fusesandinstrumentcleaning. Foranyothermaintenanceoperationcontactyour
localsupplier.
Cleaning and General Care
ExternalCleaning:
o
o
o
Switchofftheinstrumentanddisconnectthepowersupplycord.
Useasoftdampclothtocleanallexternalsurfaces.
Amild liquid detergent (e.g. Decon) may beused toremove stubborn marks.
SampleCompartmentSpillages:
o
o
o
o
o
Switchofftheinstrumentanddisconnectthepowersupplycord.
Thesamplecompartmentiscoatedinachemicalresistantfinish. However,
strongconcentrationsofsamplemayaffectthesurfaceandspillagesshouldbe
dealtwithimmediately.
Asmalldrainholeinthesamplecompartmentallowsexcessliquidtodrain
awayontothebenchortablefromundertheinstrument.
Use a soft dry cloth to mop out the sample compartment.
Reconnectthepowersupplycordandswitchontheinstrument.
Fuse Replacement
Selecttheappropriatefusesforyourlocalsupply. Twoidenticalfusesneedtobe
loaded. For LO 100-120V operation use 2 x 1.25A T fuses and for
HI 200-240V operation use 2 x 630mA T fuses.
o
o
o
o
o
18
Switchofftheinstrumentanddisconnectthepowersupplycord. Thefuse
holder can only be opened if the power supply plug has been removed.
Thefuseholderislocatedbetweenthepowerinputsocketandtheon/off
switchonthebackpaneloftheinstrument.
Slideopenthefuseholderbypullingatthenotch.
Placefusesintothefuseholderandslidebackintoposition.
Reconnectthepowersupplycordandswitchontheinstrument.
English
Issue 03 - 01/2000
Deuterium Lamp Replacement
Replacementlampsareavailablefromyourlocalsupplier.
Thedeuteriumlampbecomesveryhotinuse,soallowatleast10
minutesbeforechanging. Careshouldbetakennottotouch theoptical
surfaceofthenewlampwithyourfingers;iftouched,theareashouldbe
cleanedwithmethanol.
o
o
o
o
o
o
o
o
Switchofftheinstrumentanddisconnectthepowersupplycord.
Releasetheinstrumentcoverbyunscrewingthesevenscrewsinthebase.
Carefullylifttopcoverupwards,tiltandplaceontherightsideofthe
instrumenttakingcarenottodamagetheribboncables.
Depresscatch'A'andliftconnectorawayfromcircuitboard.
Remove two screws 'B'.
Lift deuterium lamp andbracket assembly away from mounting plate.
Placenewdeuteriumlampandassemblyintoposition,locatingpins'C'into
holesandslotinmountingplate.
Refittwoscrews'B'andtighten.
Refitconnector'A'pushingdownwardsuntilthecatchsnapsshut.
Refittheinstrumenttopcover,takingcarenottotraptheribboncable.
Refitthesevenscrewsinthebase.
Reconnectthepowersupplycordandswitchontheinstrument.
Issue 03 - 01/2000
English
19
SPECIFICATIONS
Lightsource
Wavelength range
Wavelengthcalibration
Wavelengthaccuracy
Wavelengthreproducibility
Bandwidth
Straylight
Photometricreproducibility
Photometricrange
Photometriclinearity
Cell size accommodated
Dimensions
Weight
Environmentalconditions
Safetystandard
EMC standard
Qualitysystem
DigitalOutput
Electricalspecifications
Externalfuseratings
Internalfuseratings
VA rating
Voltage
Frequency
Deuterium arc lamp
Fixed at 230, 260, 280 and 320 nm
Factoryset
±2 nm
Betterthan±0.1nm
5 nm
Less than 0.1%T at 320 nm with NaNO2
0.5% of Abs reading or 5mA which ever is the
greater
0 to 3.000 Abs
±1% of reading or ± 0.005 A to 3 A, whichever
isthegreater
0.5mmpathlengthcapillarycell
1 mm, 5 mm and 10 mm pathlength
standardcell
270 (w) x 320 (d) x 130 (h)
3.5kg
Indoor use only, away from inflammable
materials
Temperature 5 oC to 40 oC
Maximum relative humidity 80 % up to 31 oC
decreasinglinearlyto50%at40oC
InstallationCategoryII
IEC 1010
CISPR 22
Designedand manufacturedin accordancewith
an ISO 9001 approved quality system
Centronicsparalleloutput(printerversiononly)
100-120V - 1.25 A T
200-240V - 630 mA T
F101 - 3.15 A T
F102 - 2 A T
100 VA
100 - 120 V
+ 10 %
200 - 240 V
50/60 Hz
}
Specificationsaremeasuredaftertheinstrumenthaswarmedupatconstant
ambienttemperatureandaretypicalofaproductionunit.
Aspartofourpolicyofcontinuousdevelopmentwereservetherighttoalter
specificationswithoutnotice.
20
English
Issue 03 - 01/2000